检出限
胶体金
微分脉冲伏安法
多巴胺
碳纳米管
血清素
纳米材料
材料科学
化学
纳米颗粒
电极
纳米技术
色谱法
循环伏安法
电化学
医学
内科学
生物化学
受体
物理化学
作者
Truţă Florina,Mihaela Tertiş,Cécilia Cristea,Graur Florin
出处
期刊:Current Analytical Chemistry
[Bentham Science]
日期:2021-03-11
卷期号:17 (3): 374-384
被引量:3
标识
DOI:10.2174/1573411016999200518084746
摘要
Background: Neurotransmitters are chemical messengers with crucial implication in the human body. Perturbations in the concentration of neurotransmitters can affect a multitude of mental and physical functions such as heart rate, sleep, appetite and mood. Thus, the sensitive detection of these compounds is a real need for a new generation of treatments. Methods: Simultaneous detection of two important neurotransmitters, namely dopamine and serotonin, was investigated in this study using differential pulse voltammetry. The optimization of several surface parameters was performed in order to choose the best electrode material for electrochemical oxidation of targets. Screen-printed electrodes based on carbon, gold and platinum and modified with different nanomaterials (carbon nanotubes, gold nanoparticles and carbon nanotubes decorated with gold nanoparticles) were tested. Results: Carbon-based electrodes modified with multiwall carbon nanotubes and gold nanoparticles were chosen after the optimization protocol. Linear correlations between the analytic signals obtained and the concentration of dopamine and serotonin, respectively were obtained with good sensitivity and the detection limits were 0.3 μM for dopamine and 0.8 μM for serotonin with no significant reciprocal influences. Selectivity studies were also performed, as well as tests in real samples (e.g., human serum, tears and saliva) complex matrices for which acceptable recoveries were obtained. Conclusion: The results obtained in this study can be considered as an important starting point for the development of a fast and simple method for selective and highly sensitive detection of neurotransmitters, with possible applications in the diagnosis of different pathologies and for monitoring the effectiveness of the applied drug treatment.
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