中国仓鼠卵巢细胞
半乳糖
新陈代谢
生物化学
化学
碳水化合物代谢
细胞培养
生物
遗传学
受体
作者
Mauro Torres,Julio Berríos,Yandi Rigual,Yesenia Latorre,Mauricio Vergara,Alan J. Dickson,Claudia Altamirano
标识
DOI:10.1016/j.ces.2019.04.049
摘要
Chinese Hamster Ovary (CHO) cells are the main expression system for production of therapeutic recombinant (r-) proteins. The increased demand for these therapeutics has boosted the development of more robust production processes. Use of optimised feed/media has dramatically improved the performance of CHO cells in culture. However, such progress in biomass synthesis and r-protein production often come with an increased accumulation of lactate. In this study, we present a combined feeding strategy that uses galactose and lactate to replace glucose in CHO cell cultures. Replacement of glucose by galactose and lactate sustained cell growth and r-protein production in CHO cells. This strategy supported a better-balanced and more efficient metabolism, observed by an overall decreased consumption of carbon sources and amino acids, associated with an increased ATP production per C-mol consumed. Our results provide new insights of CHO cell metabolism in glucose-free media based on galactose and lactate.
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