奥沙利铂
细胞凋亡
结直肠癌
细胞周期
流式细胞术
药理学
细胞周期检查点
癌症研究
细胞周期蛋白D1
癌细胞
PI3K/AKT/mTOR通路
活性氧
癌症
不利影响
佐剂
化学
细胞生长
医学
免疫学
内科学
生物化学
作者
Lin Yang,Bin Li,Jimin Zhao,Lulu Wei,Yuehua Wang,Mingyue Wang,Vermont P. Dia,Xianjun Meng
摘要
Abstract The use of natural compounds to potentiate the effect of drugs and lower their adverse effects is an active area of research. The objective is to determine the effect of combined blueberry extracts (BE) and oxaliplatin (OX) in colon cancer cells. The results demonstrated that treatments of BE/OX showed inhibitory effects on HCT‐116 cell and nontoxic effect on CCD‐18Co normal colon cells. Flow cytometry analysis indicated that treatment with the BE, OX or in combination could induce G0/G1 cell cycle arrest, apoptosis, increase of reactive oxygen species, and induce loss of mitochondrial membrane potential in HCT‐116 cells. Furthermore, after treatments, the expression of inflammatory cytokines was decreased, cyclin D1 and CDK4 were decreased; caspases‐3 and 9 were activated; the Akt/Bad/Bcl‐2 pathway was modulated. Moreover, the combination treatment had a considerably higher growth inhibitory effect on human colon cancer HCT‐116 cells than that of BE or oxaliplatin alone. Our results showed that BE increased the anticolon cancer effect of OX making it an attractive strategy as adjuvant therapy to potentially reduce the adverse side effects associated with chemotherapeutic drugs.
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