Several 5- and 7-substituted isatins were prepared from corresponding anilines or unsubstituted isatin. Some novel substituted isatin (2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl)thiosemicarbazones were synthesized by reaction of 2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyl thiosemicarbazide with these isatins. The reaction was carried out by using microwave-assisted heating method.