NG701 inhibition on formation of foam cell via down-regulating CD36 mRNA expression

Objective: To investigate the effect of NG701 on the formation of foam cell and the expression of CD36 mRNA. Methods: Macrophages were incubated with ox-LDL to establish the foam cell model. Different concentration of NG701 were given for intervention. The cellular lipid accumulation was examined by oil red staining. The cellular contents of total cholesterol (TC) and cholesterol ester (CE) were detected by enzymic methods. The levels of CD36 mRNA were determined by RT-PCR. Results: NG701 could significantly decrease the content of TC, CE, and CE/TC in foam cell in middle and high dose groups. RT-PCR assays showed that the expression of CD36 mRNA was down-regulated in a dose-dependent manner. Conclusion: NG701 could inhibit the formation of foam cell via down-regulating CD36 mRNA expression.