单加氧酶
细胞色素P450
特种化学品
合成生物学
区域选择性
计算生物学
异源表达
代谢工程
生化工程
生物
化学
生物化学
酶
基因
重组DNA
有机化学
催化作用
工程类
作者
Yifeng Wei,Ee Lui Ang,Huimin Zhao
标识
DOI:10.1016/j.cbpa.2017.08.006
摘要
Cytochrome P450 monooxygenases (P450s) catalyze regioselective and stereoselective oxidative modifications of a wide variety of substrates, and are involved in the biosynthesis of many natural products. Despite the complex requirements of the P450 reaction system and its intransigence to recombinant expression, the promise of harnessing P450 reactivity for the industrial-scale production of specialty chemicals has led to much effort invested in P450 engineering. Here we review recent developments (between 2015 and 2017) in the application of P450s and their engineered variants as biocatalysts. We describe strategies for the reconstitution of P450 activity in heterologous microbial hosts, and the expanding repertoire of non-natural reactions catalyzed by engineered P450s.
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