上皮-间质转换
人绒毛膜促性腺激素
生物
癌症研究
转移
结直肠癌
内科学
癌症
内分泌学
医学
激素
作者
Futoshi Kawamata,Hiroshi Nishihara,Shigenori Homma,Yasutaka Kato,Masumi Tsuda,Yuji Konishi,Lei Wang,Shinji Kohsaka,Cheng Liu,Tadashi Yoshida,Mishie Tanino,Shinya Tanaka,Hideki Kawamura,Toshiya Kamiyama,Akinobu Taketomi
标识
DOI:10.1016/j.ajpath.2017.08.034
摘要
Ectopic production of free β human chorionic gonadotropin (hCGβ) has been associated with aggressive behavior in non-trophoblastic tumors. hCGβ shares common evolutionary sequences with transforming growth factor-β (TGF-β), which represents a major driving force of epithelial-to-mesenchymal transition (EMT). In this study, we examined the biological roles of hCGβ during EMT and its clinical significance in colorectal cancer (CRC) progression. Eighty CRC specimens and 54 preoperative serum samples were analyzed. hCGβ-overexpressing human CRC cell lines were examined for invasiveness and tumorigenicity, and the expression of EMT-associated genes was investigated. In human CRC, histologic hCGβ positivity [13/80 (16.3%)] was lower than serologic hCGβ positivity [13/54 (24.1%)]. However, it was significantly correlated with several clinicopathological features and unfavorable outcome (P < 0.05). hCGβ-overexpressing cell lines had increased invasiveness, migratory ability, and metastatic potential in mice (P < 0.01). Western blot, PCR, and microarray analyses showed hCGβ altered expression of EMT-related genes, including E-cadherin, phosphorylated SMAD2, SNAIL, and TWIST. hCGβ-induced SNAIL and TWIST overexpression levels were reversible by type I and type II TGF-β receptor inhibitors (P < 0.05). hCGβ thus induces EMT via the TGF-β signaling pathway, and it may represent a molecular target in CRC treatment.
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