[Analysis and Confirmation of the Ambiguous Results from HLA-DRB1 Genotyping Based on PacBio Sequencing].

OBJECTIVE: genotyping in one case. METHODS: locus were further analyzed and confirmed through PacBio SMRT third-generation sequencing. RESULTS: *12:10. CONCLUSION: For some new alleles suggested by NGS, especially the ambiguous ones that are far away from other heterozygous sites, it is necessary to analyze and confirm them by other methods such as the third-generation long-read sequencing technology to obtain reliable results.