衰老
氧化应激
超氧化物歧化酶
内皮功能障碍
活性氧
内皮干细胞
炎症
内皮
丙二醛
免疫学
医学
细胞生物学
生物
化学
内科学
生物化学
体外
作者
Zhefeng Wang,Li-Lin Li,Souqi Liao,Haitao Ren,Yong Jiang,Fei Jia,Liang Cai,Keda Zhang
标识
DOI:10.31083/j.fbl2812367
摘要
Background: Vascular endothelial dysfunction is an early phenotype of aging-related vascular dysfunction. Delaying vascular aging and preventing cardiovascular disease are major public health problems that urgently need to be solved. Scientists have studied various drugs to prevent the occurrence and progress of cardiovascular disease, but progress has been slow. Here, the antisenescence and anti-endothelial damage of canthaxanthin (CX, which is an active molecule from food) has been studied. Methods: This study was performed by adding CX to a model of cell senescence and oxidative damage induced by hydrogen peroxide. Cellular senescence markers (e.g., p16, p21, and p53) and oxidative damage markers (e.g., reactive oxygen species, nitric oxide, malondialdehyde, superoxide dismutase) were evaluated by the enzyme-linked immunosorbent assay, laser scanning confocal microscopy, and Western blotting. Results: We found that CX downregulated the expression level of senescence-associated molecules, and significantly reduced the oxidative damage of vascular endothelial cells. These observations showed that CX effectively alleviated the senescence of vascular endothelial cells. Furthermore, CX treatment reduced the expression levels of interleukin-6 (IL-6), tumor necrosis factor alpha, and IL-1β. Finally, in vivo, CX significantly alleviated vascular senescence. Conclusions: The current study shows that CX has potential application value for treating vascular aging or endothelial cell senescence.
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