质粒
枯草芽孢杆菌
营养不良
拉伤
杆菌科
芽孢杆菌目
生物
微生物学
大肠杆菌
细菌
遗传学
DNA
基因
解剖
作者
Mitsuhiro Itaya,Mitsuru Sato,Satoru Watanabe,Masakazu Kataoka
摘要
In this study, a Bacillus natto strain named NEST141 was constructed. The strain carries no plasmids and is an authentic proline auxotroph-a feature that confers effective selection conditions for plasmids transferred from a donor, such as Bacillus subtilis 168, via a pLS20-based conjugational transfer system. We have provided a standard effective protocol for the delivery of plasmids larger than 50 kilobase pairs. These results indicate that the B. natto NEST141 strain can become a standard model, like B. subtilis 168, for extensive genetic engineering with diverse applications.
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