环介导等温扩增
化学
检出限
病菌
清脆的
回文
基因组DNA
DNA
生物传感器
计算生物学
胶体金
纳米技术
色谱法
微生物学
基因
生物
生物化学
纳米颗粒
材料科学
作者
Qiushi Wang,Yihua Ren,Tian Meng,Xin Yang,Lin Li,Yang Hao,Hongwei Hou,Masoud Negahdary,Yi Wan,Fengge Song,Jinghong Li
出处
期刊:Talanta
[Elsevier]
日期:2024-03-01
卷期号:269: 125458-125458
标识
DOI:10.1016/j.talanta.2023.125458
摘要
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas enzymes have been widely applied for biosensor development, combined with various isothermal amplification strategies (IAS) to boost sensitivity and specificity. Currently, the unstable assay and tedious manipulation usually hinder its practical applications. Here, a Cas14a1-advanced LAMP assay (CALA) combined with Rapid Extraction of Bacterial Genomic DNA (REBGD) is proposed for pathogen detection. For rapid CALA, a single stranded fluorescence reporter and ssDNA-gold nanoparticles (AuNPs) are used as signal indicators to establish ultrasensitive and visual platforms. This assay displays precise detection of bacteria, which can achieve an ultrasensitive limit of detection (LOD) 10 aM target genomic DNA. Furthermore, the high reliability of pathogen diagnostic for contrived samples is validated through the rapid visual CALA platform, demonstrating the promising practical testing availability of pathogen detection.
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