Salylic acid minimize cadmium accumulation in rice through regulating the fixation capacity of the cell wall to cadmium

Although salylic acid (SA) has been linked to how plants react to cadmium (Cd) stress, the exact mechanism is still unknown. The endogenous SA concentration in the rice (Oryza sativa L.) roots was enhanced by Cd stress in the current investigation, and exogenous SA reduced the hemicellulose content in root cell wall, which in turn inhibited its Cd binding capacity. What's more, exogenous SA also decreased the transcription level of genes such as Natural Resistance-Associated Macrophage Protein 5 (OsNRAMP5) and a major facilitator superfamily gene-OsCd1 that responsible for root Cd absorption. Finally, less Cd was accumulated in the rice as a result of the higher expression of Heavy Metal ATPase 3 (OsHMA3), Cation/Ca exchanger 2 (OsCCX2) and Pleiotropic Drug Resistance 9 (OsPDR9/OsABCG36) that were responsible for separating Cd into vacuole and getting Cd out of cells, respectively. In contrast, mutant with low SA level accumulated more Cd. Additionally, SA enhanced endogenous nitric oxide (NO) levels, and its alleviatory effects were mimicked by a NO donor, sodium nitroprusside (SNP). In conclusion, SA enhanced rice's Cd resistance through regulating the binding capacity of the cell wall to Cd, a pathway that might dependent on the NO accumulation.