碱性磷酸酶
免疫分析
化学发光
纳米技术
化学
比色法
色谱法
材料科学
酶
生物化学
生物
抗体
免疫学
作者
Lin Liu,Yong Keun Chang,Jiaxin Lou,Shuo Zhang,Xinyao Yi
出处
期刊:Molecules
[MDPI AG]
日期:2023-09-11
卷期号:28 (18): 6565-6565
被引量:1
标识
DOI:10.3390/molecules28186565
摘要
The drive to achieve ultrasensitive target detection with exceptional efficiency and accuracy requires the advancement of immunoassays. Optical immunoassays have demonstrated significant potential in clinical diagnosis, food safety, environmental protection, and other fields. Through the innovative and feasible combination of enzyme catalysis and optical immunoassays, notable progress has been made in enhancing analytical performances. Among the kinds of reporter enzymes, alkaline phosphatase (ALP) stands out due to its high catalytic activity, elevated turnover number, and broad substrate specificity, rendering it an excellent candidate for the development of various immunoassays. This review provides a systematic evaluation of the advancements in optical immunoassays by employing ALP as the signal label, encompassing fluorescence, colorimetry, chemiluminescence, and surface-enhanced Raman scattering. Particular emphasis is placed on the fundamental signal amplification strategies employed in ALP-linked immunoassays. Furthermore, this work briefly discusses the proposed solutions and challenges that need to be addressed to further enhance the performances of ALP-linked immunoassays.
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