Arthrobacter horti sp. nov., isolated from mountain soil

Gram-stain-positive, aerobic, rod-shaped strains, YJM1 T and YJM12S, were isolated from Maebong Mountain, Dogok-dong, Gangnam-gu, Seoul, Republic of Korea. Strains YJM1 T and YJM12S exhibited growth at 5–35 °C (optimum, 20–30 °C) and pH 6–9 (optimum, pH 7) and in 0–4 % (w/v) NaCl. Strains YJM1 T and YJM12S showed highest 16S rRNA gene sequence similarity to the following members of the genus Arthrobacter : A. nanjingensis A33 T (98.3 %/98.2 % similarity), A. woluwensis NBRC 107840 T (98.2 %/98.1 %), A. humicola KV-653 T (97.3 %), A. oryzae KV-651 T (97.3 %), and A. globiformis NBRC 12137 T (97.2 %). The strains grew well on Reasoner's 2A, nutrient, Mueller–Hinton, yeast–dextrose, and glucose–peptone–meat extract agars. The major polar lipids of strain YJM1 T were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylinositol. The primary respiratory quinone of strain YJM1 T was MK-9(H 2 ), and the major fatty acids of strains YJM1 T and YJM12S were anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 , and iso-C 16 : 0 . The DNA G+C content, based on the whole genome sequence of strain YJM1 T , was 68.3 mol%. Average nucleotide identity values and digital DNA–DNA hybridization values between strain YJM1 T and the reference strains ranged from 75.0 to 92.7 % and from 21.0 to 65.3 %, respectively. Strain YJM1 T exhibited antimicrobial activity against Bacillus subtilis and Escherichia coli . Considering the chemotaxonomic, phenotypic, genotypic, and phylogenetic results, we propose the strain YJM1 T represents a novel species in the genus Arthrobacter and suggest the name Arthrobacter horti sp. nov. (type strain YJM1 T =KACC 23300 T =JCM 36483 T ).