Development of molecularly imprinted nanoparticles for the detection of cardiovascular diseases biomarker Angiotensin II in human serum

Abstract Angiotensin II (Ang II) is a peptide hormone that causes vasoconstriction and an increase in blood pressure. Due to its relationship with cardiovascular diseases, it is an important biomarker in blood serum. In this study, Ang II imprinted nanoparticles were synthesized by miniemulsion polymerization reaction for the determination of Ang II from human serum. Hydroxyethyl methacrylate (HEMA) based Ang II imprinted (Ang II‐MIP np ) and non‐imprinted nanoparticles (NIP np ) were synthesized, characterized by zeta size analysis, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR‐ATR). The average particle size of the NPs was recorded as 50 nm. Ang II molecules were successfully removed from the Ang II‐MIP np with a 98% success rate using 0.5 M NaCl solution to obtain template‐specific cavities. Then, the adsorption studies were achieved. The binding capacity was found as 4500 pg. g −1 at 700 pg. mL −1 Ang II concentration. The selectivity studies showed that Ang II‐MIP np can recognize Ang II molecules 2.76 times and 3.23 times selectivity than Ang I and Vsp respectively. Reusability studies shows that the synthesized nanomaterial is reusable.