Experimental approaches to evaluate solid lipid nanoparticle-based drug delivery systems

Solid lipid nanoparticles (SLNs) are potential drug carriers due to the several advantages they offer. The physicochemical stability of lipid carriers varies significantly due to their diverse compositions and structures. Appropriate analytical methods are required for the complete characterization of SLNs. Physicochemical characterization includes analysis of bulk properties like particle size, size distribution, zeta potential, morphology, stability, polymorphism, crystallinity, and molecular level properties like microenvironments within nanoparticles and their interactions with drugs. Moreover, drug loading, drug entrapment efficiency, and drug release kinetics are essential parameters to evaluate the efficacy of SLNs as drug delivery systems. In addition to testing the physicochemical stability and functionality of SLN formulations, it is essential to investigate their desired actions through in vivo studies, which are beyond the scope of this article. This review briefly discusses the different experimental techniques and their applications in the field of solid lipid nanoparticles. These techniques can also be used to characterize nanostructure lipid carriers, which are second-generation lipid nanoparticles.