In vitro regeneration and optimization of physical and chemical mutagenesis protocol in tuberose ( Agave amica (Medik.) Thiede & Govaerts) cv. ‘Arka Vaibhav’

Purpose Tuberose (Agave amica [Medik.]) is a vegetatively propagated commercial flower crop with limited genetic variability. Crossing barriers prevailing in tuberose necessitates modern breeding techniques like in vitro mutagenesis to generate variability. Hence, this study aimed to establish an efficient in vitro regeneration protocol for the rapid multiplication of tuberose and optimize the method for in vitro mutagenesis using the terminal stem scale as the explant.