Location of Tetrachlorsalicylanilide Taken up by Bacillus megaterium

SUMMARY: 14C-Tetrachlorsalicylanilide (TCS) was taken up by Bacillus megaterium, becoming localized on the cytoplasmic membrane and was recoverable from the membrane by treatment with normal horse serum. Normal phase-dark B. megaterium protoplasts became phase-bright and distorted on addition of TCS; osmotically resistant ‘protoplasts’ in buffer were obtained from TCS-treated B. megaterium subsequently treated with lysozyme. Both these conditions were inhibited or annulled by serum.