First Report of Streptomyces bottropensis Causing Potato Common Scab in Hebei Province, China

HomePlant DiseaseVol. 101, No. 3First Report of Streptomyces bottropensis Causing Potato Common Scab in Hebei Province, China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Streptomyces bottropensis Causing Potato Common Scab in Hebei Province, ChinaBo Zhou, M. S. Zhang, and X.-K. MaBo ZhouSearch for more papers by this author, M. S. ZhangSearch for more papers by this author, and X.-K. MaSearch for more papers by this authorAffiliationsAuthors and Affiliations Bo Zhou M. S. Zhang , Shandong Agricultural University, Taian, 271018, Shandong, China; and X.-K. Ma , Shaanxi Normal University, Xi’an 710055, Shaanxi, China. Published Online:20 Dec 2016https://doi.org/10.1094/PDIS-05-16-0671-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Potato common scab (PCS) is caused by phylogenetically diverse Streptomyces spp. To identify new pathogens, potato tubers (Solanum tuberosum cv. Favorita) with scab symptoms were collected from fields in Hebei Province in November 2014. An isolate was obtained by transferring single colonies and incubation at 28°C on Streptomyces selective medium (STR). Morphological, cultural, physiological, and biochemical characteristics suggested that the isolate belongs to the genus Streptomyces. BLAST analysis of the sequences of its 16S rRNA amplicon (GenBank accession no. KU189257) against the NCBI GenBank database determined that the isolate was highly related to Streptomyces bottropensis type strain ATCC25435 (∼100%), and it was designated as S. bottropensis AR (ACCC1953). The isolate gave a positive PCR reaction with primers BottF and BottR (Wanner 2006) for S. bottropensis, but had negative reactions with primers for individual S. scabies, S. stelliscabiei, S. acidiscabies, S. turgidiscabies, and S. aureofaciens, reported as pathogenic species to potato (Lehtonen et al. 2004; Wanner 2006). PCR amplification and sequencing revealed that all three pathogenicity-related genes involved in PCS were found in this isolate, with high similarity (99 to 100%) with those in S. scabies ATCC49173 (txtAB, EU864252; nec1, AM253591; and tomA, FJ007481). However, these genes were not detected in the type strain of S. bottropensis. Potato (cv. Favorita) tubers were incubated in sterilized potting mix in a growth chamber at 25 to 28°C until the seed germinated. Each potato seedling was transferred to a new pot in a greenhouse. To complete Koch’s postulates, 2 weeks later, the potting mix was infested with S. bottropensis AR (106 CFU/cm3) with five pots. Potting mix with suspensions with S. bottropensis or S. scabies (106 CFU/cm3) were used as negative and positive controls, respectively. The experiment was done twice. Three months later, potato tubers inoculated with either S. bottropensis AR or S. scabies exhibited typical scabby symptoms, but there were no symptoms in the potting mix infested with S. bottropensis (the type strain). The pathogen was reisolated from the lesions and confirmed identical to the original isolate by DNA sequences. The scab symptoms associated with S. bottropensis AR were very similar to those caused by reported Streptomyces pathogens (superficial, slightly raised, pitted, or polygonal lesions). To our knowledge, this is the first report of S. bottropensis causing PCS; other formal reports include S. turgidiscabiei, S. acidiscabiei, and S. galilaeus in China (Guo et al. 2014; Yu et al. 2015; Zhao et al. 2010). Pathogenicity of Streptomyces spp. may be transferred through horizontal gene transfer of pathogenicity genes, and may explain the emergence of new pathogenic S. bottropensis in China.References:Guo, F. L., et al. 2014. 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Plant Pathol. 59:405. https://doi.org/10.1111/j.1365-3059.2009.02126.x Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 3 March 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 9 Feb 2017Published: 20 Dec 2016First Look: 9 Nov 2016Accepted: 31 Oct 2016 Pages: 502-502 Information© 2017 The American Phytopathological SocietyCited byStreptomyces caniscabiei sp. nov., which causes potato common scab and is distributed across the worldInternational Journal of Systematic and Evolutionary Microbiology, Vol. 72, No. 1Biocontrol of potato common scab by Brevibacillus laterosporus BL12 is related to the reduction of pathogen and changes in soil bacterial communityBiological Control, Vol. 153A Novel Species-Level Group of Streptomyces Exhibits Variation in Phytopathogenicity Despite Conservation of Virulence LociAlexandra J. Weisberg, Charles G. Kramer, Raghavendhar R. Kotha, Devanand L. Luthria, Jeff H. Chang, and Christopher R. 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