Studies on the compatibility principles of panax notoginseng saponins with borneol using a novel simulating system

[Objective] The three main components of panax notoginseng saponins(ginsenoside Rg1,Rb1 and notoginsenoside R1) as quality control components,using a drug dissolution/absorption simulating system to study the change of the membrane permeability of panax notoginseng saponins after compatibility with borneol and study its possible mechanism.The system will be used to study traditional Chinese medicine,to provide a new method for the development of traditional Chinese medicine.[Methods] HPLC method was established to determine the three main constituents of PNS(ginsenoside Rg1、Rb1 and notoginsenoside R1) simultaneously.DDASS was used to study the changes of cumulative permeation amount and the apparent permeability coefficients(Papp) of PNS after compatibility with borneol.Variance analysis was used to analyze the differences between the mean values of Papp by SPSS.[Results] Papp values of ginsenoside Rg1,Rb1 and notoginsenoside R1 respectively were(0.49 ± 0.09) cm/s,(0.07 ± 0.03) cm/s,(0.58 ± 0.04) cm/s,and it was significantly increased after compatibility with borneol 2.4、2.2 and 2.7 times,respectively(P0.05).The Papp values of Rg1,Rb1 and R1 after compatibility with CsA were(0.66 ± 0.07) cm/s,(0.14 ± 0.05) cm/s,(0.92 ± 0.27) cm/s.There were no significant difference(P0.05) compared with PNS group.[Conclusion] Borneol can increase the membrane permeability of the PNS(ginsenoside Rg1,Rb1 and notoginsenoside R1),and it can promote PNS oral absorption and enhance its bioavailability.CsA does not improve the PNS membrane permeability.Rg1,Rb1 and R1 maybe not the substrate of P-glycoprotein.The mechanism of enhancing permeation by borneol maybe influence by the paracellular route transport.