Analysis of content differences and variation trends of the principal component isomers and related substances in the four generations of glycyrrhizin preparations

Objective: To analyze the content differences and variation trends of the principal component isomers and related substances in the four generations of different glycyrrhizin preparations on the domestic and foreign markets. Methods: The condition of sample pretreatment was investigated,and the HPLC analytical method was established for the simultaneous separation and detection of 18α- glycyrrhizic acid,18β- glycyrrhizinic acid,related substance A and related substance B of ten brands of glycyrrhizin preparations. The labeled amount and composition ratio of 18α- Gly and 18β- Gly of glycyrrhizin preparations were calculated. The content differences and variation trends of each component in glycyrrhizin preparations were analyzed. Results: The labeled amount of ten brands of glycyrrhizin preparations accorded with the specification in the Chinese Pharmacopoeia. The content of principal components and related substances and the composition ratio of 18α- Gly and 18β- Gly showed significance difference among different brands of glycyrrhizin preparations. The total amount of each component in imported glycyrrhizin preparations was higher than that in domestic products. Conclusion: The method is simple,sensitive,and reproducible with accurate and reliable results,which can be used for content detection of various dosage forms of glycyrrhizinate and quality control. The analysis results can provide reference for the selection of clinical medicine and new- medicine development.