Evaluation of modified reticulocyte staining solution

Objective To evaluate a kind of modified reticulocyte(Ret) staining solution.Methods The observation test of the modified Ret staining solution was performed under the microscope,counted the staining time,analyzed the effective life and tested its accuracy and precision.Results The modified Ret staining samples had a lot of characters,such as not easily fade,few residues,clear visual field and bright background,etc.Compared with the routine staining solution-brilliant cresyl blue,the staining time was short(5 min) and the effective life was long(30 months).The accuracy of the modified staining solution was consistent with that of the brilliant cresyl blue(P 0.05),but the precision of the modified staining solution was better than that of the brilliant cresyl blue.Conclusion The modified Ret staining solution is an excellent staining.It is worthy to be spreaded in practice.