Objective To observe the inhibition effect of the extract B of Polygonatum odoratum(EB-PAOA) on human cervix cancer Hela cells.Methods Cervix cancer Hela cells were cultured in vitro and then different concentration of EB-PAOA were added to Hela cells.The growing curve was drawn.MTT assay was adopted to determine the inhibition rate to Hela cells.Results EB-PAOA can inhibit the proliferation of Hela cells,and the inhibition rate is of time and dose dependent.Conclusions EB-PAOA has obvious inhibition effect on Hela cells.