With 25 10-basic primers, the genomes DNA of three groupes of diploid and tetraploid Antirrhinum majus were randomly amplified. The results showed the fingerprinting of diploid Antirrhinum majus and that of its autotatraploid were different with cultivars and primers; The DNA sequences were changed besides polyploided during polyploided inducement on Antirrhinum majus. So the abundant germresource was offered for breeding new cultivars. The fingerprinting can offer molecular evidence for identifying new cultivars of Antirrhinum majus and establish the basis for protecting new cultivars.