Expression and purification and biological activicty assay of recombinat human TRAIL protein

E.coli BL21 baring a expression plasmid of human TRAIL protein was induced for expression with IPTG.The protein expressed in a form of inclusion bodies and was purified through cell disruption, inclusion bodies extraction, solubilization renaturation, and a combination of Ni-NTA Superflow affinity and ion-exchange chromatography. The result of SDS-PAGE and Western blot demonstrated that the purified protein was a single band with molecular weight of 19kD and reacted specially with anti-human TRIAL IgG. Cytotoxic activity assay showed that the purified TRAIL protein had good antitumor activity in vitro.