The Effect of PP_(333) on Conservation in vitro of Pueraria lobata

ObjectTo study the effects of PP_(333) on preservation in vitro of Pueraria lobata.MethodsThe effect of PP_(333) on preservation in vitro of Pueraria lobata were explored by room temperature and 4℃ low temperature culture methods.Results At room and low temperature,PP_(333) could improve revival rates of Pueraria lobata plantlets.At room temperature,the treatment effect of PP_(333) with 2 mg/L 6-BA and 1 mg/L NAA was better than that of PP_(333) alone.The best PP_(333) concentrations of Pueraria lobata were 8 mg/L(treated by PP_(333) with 2 mg/L 6-BA and 1 mg/L NAA)and 5 mg/L(treated by PP_(333) alone) respectively,its revival rate was up to 80% after 180 days;At 4℃ low temperature,its treatment effect of PP_(333) was better than that of PP_(333) at room temperature.The best PP_(333) concentrations of Pueraria lobata was 5 mg/L(treated by PP_(333) with 2 mg/L 6-BA and 1 mg/L NAA),its revival rate was up to 98% after 180 days.The plantlets were cultured at room temperature that transferred to subculture medium after conserved in vitro,Pueraria lobata regenerated plantlets were as same as the normal subculture plantlets in morphology.Conclusion The suitable concentration of PP_(333) with 4℃ low temperature can improve evidently revival rates of Pueraria lobata plantlets.