Determination of Hesperidin and Naringin in Fructus Aurantii Immaturus and Fructus Aurantii by Reversed-Phase High Performance Liquid Chromatography

An HPLC method was established for the determination of hesperidin and naringin in the Fructus Aurantii Immaturus and Fructus Aurantii from different places. Hesperidin and narigin were separated on a Hypersil ODS1 column with acetonitrile05% acetic acid (volume ratio of 22∶78) as the mobile phase and detected at 283 nm. The flow rate was 10 mL/min. The column temperature was set at 35 ℃. The calibration curves were linear in the ranges of 0060 g/L-0604 g/L and 0012?5 g/L-0125 g/L for naringin and hesperidin respectively. The average recoveries were 971% for naringin and 953% for hesperidin. The method is simple, rapid, accurate and reliable.