小RNA
登革热病毒
登革热
病毒学
复制(统计)
生物
病毒复制
病毒
细胞生物学
遗传学
基因
作者
Weitao Wen,Zhenjian He,Qinlong Jing,Yiwen Hu,Cuiji Lin,Rui Zhou,Xiaoqun Wang,Yu‐Jih Su,Yuan Jiang,Zhenxin Chen,Jie Yuan,Jueheng Wu,Jun Li,Xun Zhu,Mengfeng Li
标识
DOI:10.1016/j.jinf.2014.12.001
摘要
Summary
Objective
It has been well recognized that microRNA plays a role in the host–pathogen interaction network. The significance of microRNA in the regulation of dengue virus (DENV) replication, however, remains unknown. The objective of our study was to determine the biological function of miR-548g-3p in modulating the replication of dengue virus. Methods
Here we report that employment of a microRNA target search algorithm to analyze the 5′ untranslated region (5′UTR) consensus sequences of DENV (DENV serotypes 1–4) led to a discovery that miR-548g-3p directly targets the stem loop A promoter element within the 5′UTR, a region essential for DENV replication. Real-time PCR was used to measure the expression levels of miR-548g-3p under DENV infection. We performed overexpression and inhibition assays to test the role of miR-548g-3p on DENV replication. The protein and mRNA levels of interferon were measured by ELISA and real-time PCR respectively. Result
We found that overexpression of miR-548g-3p suppressed multiplication of DENV 1, 2, 3 and 4, and that miR-548g-3p was also found to interfere with DENV translation, thereby suppressing the expression of viral proteins. Conclusion
Our results suggest that miR-548g-3p directly regulates DENV replication and warrant further study to investigate the feasibility of microRNA-based anti-DENV approaches.
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