交易激励
转录因子
细胞生物学
染色质免疫沉淀
生物
表皮生长因子受体
细胞周期蛋白D1
DNA结合域
核定位序列
癌症研究
分子生物学
细胞核
受体
发起人
核心
遗传学
基因表达
基因
细胞
细胞周期
作者
Shiaw Yih Lin,Keishi Makino,Weiya Xia,Angabin Matin,Yong Wen,Ka Yin Kwong,Lilly Y.W. Bourguignon,Mien‐Chie Hung
摘要
Epidermal growth factor receptor (EGFR) has been detected in the nucleus in many tissues and cell lines. However, the potential functions of nuclear EGFR have largely been overlooked. Here we demonstrate that nuclear EGFR is strongly correlated with highly proliferating activities of tissues. When EGFR was fused to the GAL4 DNA-binding domain, we found that the carboxy terminus of EGFR contained a strong transactivation domain. Moreover, the receptor complex bound and activated AT-rich consensus-sequence-dependent transcription, including the consensus site in cyclin D1 promoter. By using chromatin immunoprecipitation assays, we further demonstrated that nuclear EGFR associated with promoter region of cyclin D1 in vivo. EGFR might therefore function as a transcription factor to activate genes required for highly proliferating activities.
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