化学
水解
酶水解
半乳糖
低聚糖
酶
半乳甘露聚糖
多糖
生物化学
食品科学
作者
Honglei Jian,Li Zhu,Weiming Zhang,Daquan Sun,Jianxin Jiang
标识
DOI:10.1016/j.ijbiomac.2013.01.025
摘要
Enzymatic hydrolysis of Gleditsia sinensis gum was performed to produce manno-oligosaccharides having functional applications as dietary fiber and prebiotics. The optimum hydrolysis conditions, including enzyme loading, temperature and time, from response surface methodology were 8.1 U/g, 57.4 °C and 34.1 h, respectively. The yield of DP 1–5 oligosaccharides was 75.9% (29.1 g/L). The Michaelis–Menten kinetics and molecular weight distribution were determined. The obtained oligosaccharides were further separated by HPLC and SEC, and the galactose distribution of G. sinensis gum was elucidated. Results indicated that G. sinensis gum has potential to produce value-added oligosaccharides in food industries.
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