精子
透明带
生物
抗体
人类受精
单克隆抗体
融合蛋白
透明带糖蛋白
免疫学
精液
仓鼠
遗传学
抗原
免疫球蛋白超家族
细胞生物学
基因
男科
分子生物学
卵母细胞
胚胎
医学
重组DNA
作者
Naokazu Inoue,Masahito Ikawa,Ayako Isotani,Masaru Okabe
出处
期刊:Nature
[Springer Nature]
日期:2005-03-01
卷期号:434 (7030): 234-238
被引量:712
摘要
Representing the 60 trillion cells that build a human body, a sperm and an egg meet, recognize each other, and fuse to form a new generation of life. The factors involved in this important membrane fusion event, fertilization, have been sought for a long time. Recently, CD9 on the egg membrane was found to be essential for fusion, but sperm-related fusion factors remain unknown. Here, by using a fusion-inhibiting monoclonal antibody and gene cloning, we identify a mouse sperm fusion-related antigen and show that the antigen is a novel immunoglobulin superfamily protein. We have termed the gene Izumo and produced a gene-disrupted mouse line. Izumo-/- mice were healthy but males were sterile. They produced normal-looking sperm that bound to and penetrated the zona pellucida but were incapable of fusing with eggs. Human sperm also contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs.
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