检出限
纤锌矿晶体结构
生物传感器
化学
电化学
选择性
电极
纳米-
胶体金
纳米颗粒
核化学
材料科学
色谱法
纳米技术
结晶学
生物化学
六方晶系
催化作用
复合材料
物理化学
作者
Chi Zhang,Congcong Du,Wei Liu,Ting Guo,Ying Zhou,Hongyuan Zhou,Yuhao Zhang,Xiaozhu Liu,Liang Ma
出处
期刊:Agriculture
[MDPI AG]
日期:2022-02-01
卷期号:12 (2): 204-204
被引量:1
标识
DOI:10.3390/agriculture12020204
摘要
In this paper, an electrochemical biosensor was established for the high-sensitivity detection of Tenuazonic acid (TeA) in fruits based on the enrichment of flower-shaped nano-ZnO and the specific recognition of immune response. Herein flower-shaped nano-ZnO (ZnO NFs) with a hexagonal wurtzite structure and diameter of 700–800 nm were demonstrated to have the optimal specific surface area and outstanding conductivity, compared with different morphology, sizes, and crystal structures of nano-ZnO. Second, the ZnO NFs were used as carriers for efficiently immobilizing monoclonal antibodies to obtain antibody bioconjugates, which were anchored on the 2-mercaptobenzoic acid-modified gold electrode by amide reaction. In the presence of TeA, the monoclonal antibody could specifically recognize and bind to it, resulting in a decrease in electron transfer ability on the gold electrode surface. Finally, the electrochemical biosensor showed a range from 5 × 10−5 μg/mL to 5 × 10−1 μg/mL with a detection limit of 1.14 × 10−5 μg/mL. Furthermore, it exhibited high selectivity for TeA among other analogs, such as Altenuene (ALT) and Alternariol (AOH). Notably, the proposed strategy could be employed to monitor TeA in tomato and citrus, showing potential application prospects in practical application and commercial value.
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