代谢组
代谢物
代谢组学
大肠杆菌
生物化学
新陈代谢
平衡
蛋氨酸
化学
代谢途径
核苷酸
生物
氨基酸
基因
细胞生物学
色谱法
作者
Dušica Radoš,Stefano Donati,Martin Lempp,Johanna Rapp,Hannes Link
出处
期刊:iScience
[Elsevier]
日期:2022-07-01
卷期号:25 (7): 104503-104503
被引量:16
标识
DOI:10.1016/j.isci.2022.104503
摘要
Metabolite concentrations vary across conditions and such metabolome changes are relevant for metabolic and gene regulation. Here, we used LC-MS/MS to explore metabolite concentration changes in Escherichia coli. We measured 101 primary metabolites in 19 experimental conditions that include various nutrients and stresses. Many metabolites showed little variation across conditions and only few metabolites correlated with the growth rate. The least varying metabolites were nucleotides (e.g. UTP had 10% variation) and amino acids (e.g. methionine had 13% variation). These results show that E. coli maintains protein and RNA building blocks within narrow concentration ranges, thus indicating that many feedback mechanisms in biosynthetic pathways contribute to end-product homeostasis.
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