Physical stability and subjective efficacy study of liposome loaded with Clitoria ternatea (butterfly pea) flower extract and Eugenia caryophyllus (clove) oil

Clitoria ternatea (butterfly pea) flower and Eugenia caryophyllus (clove) oil have been traditionally used as hair growth stimulating herbal and exhibited hair growth effects in vitro.This work prepared liposome loading with butterfly pea flower extract and clove oil and studied the hair growth promoting effects on eyebrows in volunteers.The liposome of butterfly pea flower extract and clove oil was prepared by loading the extract and oil into a pro-liposome.The formation of liposome was examined using the microscopy and its physical stability was studied at 4°C, 45°C and ambient temperature (30-35°C) for 1 month.The liposome of butterfly pea flower extract and clove oil was macroscopically stable but microscopic examination showed that the liposome vesicles were larger overtime.The loaded liposome was used for eyebrow growth efficacy test in 15 volunteers.The product was applied on both sides of eyebrows twice daily for 60 days.The evaluation was based on the taken photos and the satisfaction assessment scoring from 1 to 5 (very poor-excellent).The eyebrows appeared darker and thicker after 30 day and progressively more obvious at 2 month use.Participants felt that the enhancement of eyebrow thickness and darkness was good (33.3%) to excellent (66.7%).In addition, there was no irritation present which ensure the product safety.Conclusively, this work reported a stable liposome loaded with butterfly pea flower extract and clove oil.The product was subjectively proven to intensify the thickness and darkness of eyebrows.