Preparation and performance analysis of monodisperse glycidyl methacrylate modified restricted access media‐imprinted materials

Using monodisperse poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) as the matrix, and pefloxacin template molecules, a novel restricted-access medium molecularly imprinted polymers with Bovine serum albumin crosslinked on its surface was prepared through reversible addition-fragmentation chain-transfer polymerization. Then, the obtained material was employed in dispersive solid-phase extraction to analyze the fluoroquinolones in untreated egg samples by high-performance liquid chromatography-ultraviolet detection. Adsorption performance revealed a good binding amount (40.72 mg/g), fast binding kinetics (25 min), satisfactory selectivity, and good ability to eliminate matrix interference. The reusability experiments indicated the materials have good reusable performance after repetition. Under the optimized conditions, restricted access media-molecularly imprinted polymers-dispersive solid-phase extraction was combined with high-performance liquid chromatography-ultraviolet to enrich fluoroquinolones in untreated eggs, good limit of detection (1.31-3.15 μg/L) and high recovery (89.5-96.8%) were obtained. The results showed that the prepared restricted-access material is promising for the direct detection of antibiotics in complex samples.