辣根过氧化物酶
酶
化学
生物化学
核酸
固定化酶
连接器
葡萄糖氧化酶
DNA
组合化学
计算机科学
操作系统
作者
Hadi Baharifar,Kamyar Khoshnevisan,Hassan Maleki
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 151-162
被引量:1
标识
DOI:10.1007/978-1-0716-2269-8_9
摘要
The methods of compartmentalized immobilization in multi-enzyme systems containing inorganic complexes and organic scaffolds (i.e. nucleic acid (RNA and DNA), protein and lipid) have been thoroughly investigated. Compartmentalization mostly focuses on dividing individual enzyme(s) into specific location or orientation of the enzymes cooperating in cascade reaction. Organic scaffolds are preferred because of their capability for simultaneous synthesis in biological systems. Besides, the most required methods of horseradish peroxidase (HRP) and glucose oxidase (GOD) enzymes including enzyme activity measurement, enzyme immobilization, removal, and re-hybridization, and enzyme attaching have been provided because they have been extensively applied in multi-enzyme systems. Organic scaffolds have a wide range and properties. Therefore, two methods including dockerin–cohesin linker and nucleotides interaction have been demonstrated for immobilization of enzyme on protein and DNA scaffold, respectively.
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