脂肪酸去饱和酶
脂肪酸
油酸
生物
欧米茄3脂肪酸
镰刀菌
食品科学
生物化学
亚麻酸
基因
多不饱和脂肪酸
植物
六烯酸
亚油酸
作者
Muthulakshmi Chellamuthu,Selvi Subramanian,Kokiladevi Eswaran
标识
DOI:10.1016/j.sajb.2022.07.031
摘要
Alpha-linolenic acid (ALA) is the precursor for the synthesis of essential fatty acids such as EPA and DHA. The dietary requirement of omega 3 fatty acid is 1.59 g for females and 2.06 g for males. There is an urgent need for enhancing omega 3 fatty acid in vegetative sources. Sesame oil is unique in its flavor and medicinal properties but it contains only traces of ALA. In this study attempts were made to increase the omega 3 fatty acid content in sesame seeds using a bi-functional ∆12/ω3 fatty acid desaturase gene from Fusarium moniliforme. Sesame seed-specific promoter isolated from oleic acid desaturase was used for high and tissue-specific expression. The fungal bi-functional ∆12/ω3 fatty acid desaturase gene under the control of sesame seed-specific promoter was transferred to sesame the ALA content in transgenic sesame was evaluated. Expression of fungal ∆12/ω3 fatty acid desaturase gene resulted in increased ALA content up to 12 fold in transformed sesame plants compared to untransformed control.
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