Synthesis of novel anti-biofilm agent TiO2@GO for reducing the formation of Streptococcus mitis biofilm on teeth

Abstract Treatment of global infectious diseases is becoming trickier due to the biofilm formation of bacteria. A novel therapeutic approach is urgently required to address the problems posed by pathogens' persistent infections linked with biofilms. Oral biofilm caused by Streptococcus mitis may generate several dental health issues such as dental caries and periodontal disease. Here, a novel nanomaterial (TiO2@GO) had proposed as an anti-biofilm agent to hinder the formation of biofilm of S. mitis. TiO2 was synthesized by following the sol-gel method and attached with GO through the Teflon line reactor-assisted method. The interaction of TiO2@GO was confirmed through scanning electron microscopy, Fourier transforms infra-ray spectroscopy, and UV-vis spectroscopy analysis. The inhibition of biofilm was confirmed by measuring the OD of S. mitis after and before treatment through an ELISA reader and performing SEM of teeth after and before treatment. The TiO2@GO inhibited the rate of biofilm formation (89% of biofilm was reduced after 8h of treatment). The TiO2@GO can reduce 70% biofilm formation for 24h which is effective to prevent dental caries. To conclude, the proposed nanomaterial will be the potential agent to inhibit the formation of oral biofilm and treat dental caries.