检出限
分析物
电极
生物传感器
化学
信号(编程语言)
电化学气体传感器
电化学
纳米材料
电解质
纳米技术
材料科学
色谱法
计算机科学
生物化学
物理化学
程序设计语言
作者
Fa-Ting Xie,Yulong Li,Tong Yang,Yaxi Yang,Rong Hu
出处
期刊:Langmuir
[American Chemical Society]
日期:2022-09-23
卷期号:38 (39): 11828-11836
被引量:8
标识
DOI:10.1021/acs.langmuir.2c00932
摘要
Electrochemical nanotags with strong signal input are necessary for a ratiometric electrochemical sensor to overcome the drawbacks of inaccurate detection results. In this paper, the metal-organic framework (MOF) UiO-66 was utilized as an electrochemical signal tag. A stable and strong current response at +0.9 V can be detected in neutral conditions. MicroRNA (miRNA) was employed as the model analyte. Herein, an enzyme-free DNA-walker-based ultrasensitive ratiometric electrochemical biosensor in combination with Zr MOF (UiO-66) signal tags to detect miRNA was demonstrated. In the presence of miRNA, the autonomous walker movement can be initiated by miRNA, leading to the release of biotin-modified fragments. Thus, streptavidin-labeled UiO-66 nanomaterials were not bound to the electrode, generating a low signal response of UiO-66 at +0.9 V. However, the current signal of electrolyte solution as reference at +0.2 V was increased due to the enhancement of electrode conductivity. This ratiometic sensor demonstrated high sensitivity, selectivity, and reproducibility. It can eliminate the disturbance of environmental factors and basic electrode characteristics, providing more accurate signals. A limit of detection (LOD) of 0.17 fM was achieved. Moreover, the method was also used to detect miRNA-21 spiked in real serum samples.
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