Modulation of Aggregation of Covalent Organic Framework Nanosheets with Aggregation-Induced Emission Activity for Ultrasensitive No-Wash Fluorescent Immunosensor

contains lots of AIEgens, the sensitivity of the fluorescence immunosensor is expected to improve greatly. The no-wash sandwich-type immunosensor showed good performance for CA 19-9 with a linear range of 0.01 mU/mL to 10 U/mL and a detection limit of 3.3 μU/mL (S/N = 3), which was satisfactory in real samples. To the best of our knowledge, this is the first time that AIE-CONs have been used for fluorescence immunosensing, which provides a new perspective for the trace detection of tumor markers.