Simultaneous Detection of Staphylococcus Aureus and Vibrio Parahaemolyticus in Foods Using Recombinant Polymerase Amplification and Multicolored Lateral Flow Assay Based on Crispr/Cas12a

Foodborne pathogens have caused many food safety problems, which have long been serious threats to human health; thus, the detection of foodborne pathogenic microorganisms is of great importance. The current standard for foodborne pathogens detection is isolation, culture, and identification of bacteria, but this process requires a significant amount of time and is not suitable for point-of-care testing (POCT). Therefore, a rapid and efficient testing platform for foodborne pathogens (Staphylococcus aureus and Vibrio parahaemolyticus) was established here based on CRISPR/Cas12a. When combined with recombinant polymerase amplification with lateral flow assay, this process offers simultaneous detection of two targets and POCT. The test strip was constructed by different color microspheres that were labeled with different antibodies, thus visualizing the test results and avoiding cross-reactions. The entire detection process could be completed within 50 min, and the visual limit of detection was as low as 10 copies. In conclusion, a new platform was developed to simultaneously detect S. aureus and V. parahaemolyticus in foods, which demonstrated high sensitivity and accuracy. This process is promising for further applications in the detection of other foodborne pathogens.