Membrane Integrity Assay in Ferroptosis

Ferroptosis is a form of regulated cell death that relies on the accumulation of intracellular iron and subsequent oxidative stress. Ferroptotic cell death is characterized by uncontrolled lipid peroxidation, which leads to plasma membrane damage and rupture. The loss of plasma membrane integrity results in the release of intracellular components, including damage-associated molecular patterns, and can propagate death between cells in a synchronized manner. Understanding the mechanisms of ferroptotic membrane damage is crucial to comprehending this form of cell death. This chapter provides a summary of techniques for detecting plasma membrane integrity in ferroptosis, including transmission electron microscopy analysis, flow cytometry analysis, and assessments of oxidoreductase-mediated membrane damage.