小RNA
细胞外小泡
分子生物学
DNA
化学
微泡
检出限
胞外囊泡
计算生物学
生物
细胞生物学
基因
生物化学
色谱法
作者
Shuai Zhao,Shaohua Zhang,Huijun Hu,Yangchang Cheng,Kexuan Zou,Jie Song,Jinqi Deng,Lele Li,Xiaobing Zhang,Guoliang Ke,Jiashu Sun
标识
DOI:10.1002/anie.202303121
摘要
Abstract Mature microRNAs (miRNAs) in extracellular vesicles (EVs) are involved in different stages of cancer progression, yet it remains challenging to precisely detect mature miRNAs in EVs due to the presence of interfering RNAs (such as longer precursor miRNAs, pre‐miRNAs) and the low abundance of tumor‐associated miRNAs. By leveraging the size‐selective ability of DNA cages and polyethylene glycol (PEG)‐enhanced thermophoretic accumulation of EVs, we devised a DNA cage‐based thermophoretic assay for highly sensitive, selective, and in situ detection of mature miRNAs in EVs with a low limit of detection (LoD) of 2.05 fM. Our assay can profile EV mature miRNAs directly in serum samples without the interference of pre‐miRNAs and the need for ultracentrifugation. A clinical study showed that EV miR‐21 or miR‐155 had an overall accuracy of 90 % for discrimination between breast cancer patients and healthy donors, which outperformed conventional molecular probes detecting both mature miRNAs and pre‐miRNAs. We envision that our assay can advance EV miRNA‐based diagnosis of cancer.
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