电化学发光
发光体
检出限
发光
生物传感器
线性范围
材料科学
分析化学(期刊)
氧化还原
组合化学
化学
纳米技术
无机化学
光电子学
色谱法
作者
Xianzhen Song,Lu Zhao,Xiang Ren,Tao Feng,Hongmin Ma,Dan Wu,Yuyang Li,Chuannan Luo,Qin Wei
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-08-03
卷期号:7 (8): 2273-2280
被引量:16
标识
DOI:10.1021/acssensors.2c00819
摘要
Herein, we reported an efficient electrochemiluminescence (ECL) biosensor chip for sensitive detection of neuron-specific enolase (NSE). First, 3,4,9,10-perylenetetracarboxylic acid with good luminescence characteristics was used as a luminophore to obtain a stable ECL signal. Subsequently, hollow porous Co3O4/CuO concave polyhedron nanocages (CPNCs) were designed as co-reaction promoters to amplify the luminescence signals for highly sensitive trace detection of NSE. In brief, the rapid cyclic conversion of Co3+/Co2+ and Cu2+/Cu+ redox pairs could continuously catalyze the reduction of persulfate (S2O82-), thus providing a large number of essential active intermediates (SO4•-) for ECL emission. Meanwhile, the unique structure of Co3O4/CuO CPNCs possessed a large specific surface area, which greatly improved its catalytic efficiency. Third, NKFRGKYKC was developed as an affinity ligand for specific antibody fixation, which improved incubation efficiency and protected bioactivity of antibodies. Finally, we independently designed a microchip and applied it for ECL detection to improve the practical application ability of the sensor. The developed biosensor exhibited good sensitivity with a wide linear range (10 fg/mL to 100 ng/mL) and a low detection limit (3.42 fg/mL), which played an active role in the clinical application of sensing analysis.
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