Efficient nitrogen and phosphorus removal performance and microbial community in a pilot-scale anaerobic/anoxic/oxic (AOA) system with long sludge retention time: Significant roles of endogenous carbon source

Stringent wastewater discharge standards require wastewater treatment plants (WWTPs) to focus on enhancing nitrogen (N) and phosphorus (P) removal efficiency. Increasing sludge concentration by regulation of sludge retention time (SRT) would enhance wastewater treatment loads. However, phosphorus-accumulating organisms (PAOs) would be outcompeted by glycogen-accumulating organisms (GAOs) under long SRT, leading to a collapse of P removal. In this work, pilot-scale anaerobic-oxic-anoxic (AOA) and anaerobic-anoxic-oxic (AAO) systems with long SRT (30 d) were parallelly established for actual urban wastewater treatment. The results indicated that sludge reflux ratio, temperature, and C/N ratio significantly impact N and P removal performance of AOA and AAO systems with long SRT, and removal efficiency of AOA system significantly exceeded that of AAO system. AOA system with long SRT achieved the optimal performance at sludge reflux ratio of 200%, temperature of 25 °C, and C/N ratio of 8, with COD, NH₄⁺-N, TN, and PO₄^3--P removal ratio of 92.80 ± 2.24%, 97.38 ± 0.89%, 88.97 ± 2.47%, and 94.33 ± 3.27%, respectively. In addition, compared to AAO system, AOA system could save 23.08% of the aeration volume. This work highlighted that AOA system with long SRT included multiple coupled nitrogen and phosphorus removal pathways, such as autotrophic/heterotrophic nitrification, anoxic/oxic denitrification, endogenous denitrification, and denitrifying phosphorus removal. Among these, the synergistic effect of endogenous denitrification and denitrifying phosphorus removal driven by internal carbon sources contributed to satisfactory nitrogen and phosphorus removal efficiency in AOA system with long SRT.