Immobilization of protein probes on biochips with brush polymer cells

The methods of obtaining a polymer coating from polyvinyl acetate on the surface of polyethylene terephthalate polymer substrates and subsequent production by photoinduced radical copolymerization of acrylate monomers of brush polymers have been studied. Cell matrices with numerous reactive chemical groups were formed by photolithography for subsequent immobilization of proteins. Methods of activation of carboxyl groups on brush polymers attached to the surface of polyethylene terephthalate have been tested. Immobilization of the streptavidin model protein labeled with fluorescent dye Cy3 was performed to test the activation method of carboxyl groups. A variant of immunofluorescence analysis in the format of a biological microchip was tested on the streptavidin – biotinylated immunoglobulin model. Streptavidin, immobilized in brush polymer cells, retains functionality and spatial accessibility for binding to biotinylated immunoglobulin and subsequent manifestation by antibodies fluorescently labeled with Cy5 dye, which opens up prospects for the use of biological microchips with brush polymer cells on polyethylene terephthalate substrates for immunofluorescence analysis of various protein targets.