Research Process on Microbial Diversity of Producing-nitrile Hydratase and Study on Nitrile Hydratase

The reactions catalyzed by nitrile hydratase have been widely applied in organic synthesis.As an important class of catalytic agent,NHase(nitrile hydratase EC4.2.1.84) can converts nitriles to the corresponding acidamide.Because of their inherent enantio-and regioselectivities and other benefits,NHases are attractive as green,mild,and selective catalysts for setting stereogenic centers in fine-chemical synthesis and enantiospecific synthesis of a variety of acrylamides.The literature has been surveyed to provide a comprehensive coverage of the study of NHases in China at present including biodiversity of strain-producing NHase,biochemical and structural properties of NHase,strain improvement,as well as construction of gene engineering strains.Literature has also been cited to describe methods of immobilized cells and application of nitrile hydratase.