Metabolite profiling identified pipecolic acid as an important component of peanut seed resistance against Aspergillus flavus infection

In a previous study, we identified a halotolerant rhizobacterium belonging to the genus Klebsiella (MBE02) that protected peanut seeds from Aspergillus flavus infection. Here, we investigated the mechanisms underlying the effect of MBE02 against A. flavus via untargeted metabolite profiling of peanut seeds treated with MBE02, A. flavus, or MBE02+A. flavus. Thirty-five metabolites were differentially accumulated across the three treatments (compared to the control), and the levels of pipecolic acid (Pip) were reduced upon A. flavus treatment only. We validated the function of Pip against A. flavus using multiple resistant and susceptible peanut cultivars. Pip accumulation was strongly associated with the resistant genotypes that also accumulated several mRNAs of the ALD1-like gene in the Pip biosynthesis pathway. Furthermore, exogenous treatment of a susceptible peanut cultivar with Pip reduced A. flavus infection in the seeds. Our findings indicate that Pip is a key component of peanut resistance to A. flavus.