Bio Rapid Prototyping Project: Development of Spheroid Formation System for Regenerative Medicine.

Cells construct is made by spheroid-culturing the cells and collecting the spheroids. The spheroids are manufactured by dispensing cell turbid liquid into a special multiwell plate. If there is a difference in the number of cells when dispensing the cells turbid liquid, spheroids in different sizes are generated. Moreover, spheroids are not generated if the number of cells is extremely small, and if large, spheroids in distorted shapes are generated. Therefore, this study was aimed to develop a system that generates similar spheroids. In result, all the spheroids generated using developed system came in a pure spherical shape, and generation of spheroids is confirmed on all the wells.