Hydrolyzed chicken meat extract boosts the immunoregulatory effect by regulating M1/M2 Macrophage polarization

Hydrolyzed chicken meat extract (HCE) and its bioactive components (BC) decreased the activation of M1 macrophages and increased the activation of M2 macrophages in RAW264.7 cells and peritoneal macrophages. These effects of HCE and BC might be partly regulated by suppressing the MAPK and NF-B pathways and enhancing the STAT6 and Akt pathways. In addition, HCE and BC administration attenuated the oxidative stress and enhanced the antioxidant enzyme activities in RAW264.7 cells. Cyclo(Val-Pro) isolated from BC exerted clear anti-inflammatory and antioxidative effects in RAW264.7 cells. Therefore, HCE and BC boosted the immunoregulatory effect might be partly by regulating M1/M2 macrophage polarization, and might serve as potential immunoregulatory agents. • HCE and BC treatment enhance phagocytic activities of macrophages. • HCE and BC attenuate M1 macrophage activation and enhance M2 macrophage activation. • HCE and BC regulate the MAPK and NF-κB pathways and the STAT6 and Akt pathways. • HCE and BC ameliorate LPS-induced inflammation and oxidative stress in macrophages. • Cyclo(Val-Pro) exerts potent anti-inflammatory and antioxidative capacities. Hydrolyzed chicken meat extract (HCE) has previously been shown to mitigate inflammation and oxidative stress, and enhance cognitive function in humans. However, it is still not clear whether HCE and its bioactive components (BC) could affect immune function. Herein, we found that both HCE and BC administration promoted the phagocytic activity and attenuated the oxidative stress in RAW 264.7 cells. Moreover, HCE and BC treatment caused a significant decrease in M1 macrophage activation and an increase in M2 macrophage activation in RAW264.7 cells and peritoneal macrophages, resulting in an M2 dominant shift in macrophages. These effects of HCE and BC might be partly regulated by suppressing the MAPK and NF-κB pathways and enhancing the STAT6 and Akt pathways. Cyclo(Val-Pro) isolated from BC exerted clear anti-inflammatory and antioxidative effects, which might be partly responsible for the impacts of HCE and BC. Therefore, HCE and BC might serve as potential immunoregulatory agents.